Journal: MedChemComm

Article Title: Development of NMR and thermal shift assays for the evaluation of Mycobacterium tuberculosis isocitrate lyase inhibitors † †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7md00456g

doi: 10.1039/c7md00456g

Figure Lengend Snippet: (a) Isocitrate lyase catalyses the conversion of isocitrate to glyoxylate and succinate; (b) 1 H NMR spectroscopy to monitor ICL1-catalysed turnover of isocitrate into succinate; (c) corresponding plot of the isocitrate turnover data. The curve was added to aid visualisation. Sample contained 190 nM ICL1, 1 mM dl -isocitrate, 5 mM MgCl 2 and 50 mM Tris/Tris-D 11 (pH 7.5) in 90% H 2 O and 10% D 2 O. The hashtag (#) indicates Tris/Tris-D 11 peak. The errors shown are the standard deviation from three separate measurements.

Article Snippet: Using the crystal structure of ICL1 (PDB ID: ; 1F8I , resolution 2.25 Å), a screen was performed with the InterBioScreen Ltd natural product collection.

Techniques: Structural Proteomics, Standard Deviation

Journal: MedChemComm

Article Title: Development of NMR and thermal shift assays for the evaluation of Mycobacterium tuberculosis isocitrate lyase inhibitors † †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7md00456g

doi: 10.1039/c7md00456g

Figure Lengend Snippet: Kinetic parameters of ICL1 with dl -isocitrate as substrate. Measurements were made using samples contained 190 nM ICL1, varying concentration of dl -isocitrate, 5 mM MgCl 2 and 50 mM Tris/Tris-D 11 (pH 7.5) in 90% H 2 O and 10% D 2 O. The errors shown are the standard deviation from three separate measurements

Article Snippet: Using the crystal structure of ICL1 (PDB ID: ; 1F8I , resolution 2.25 Å), a screen was performed with the InterBioScreen Ltd natural product collection.

Techniques: Concentration Assay, Standard Deviation

Journal: MedChemComm

Article Title: Development of NMR and thermal shift assays for the evaluation of Mycobacterium tuberculosis isocitrate lyase inhibitors † †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7md00456g

doi: 10.1039/c7md00456g

Figure Lengend Snippet: IC 50 values of ICL1 inhibitors with dl -isocitrate as substrate. Measurements were made using samples contained 190 nM ICL1, 1 mM dl -isocitrate, 5 mM MgCl 2 , varying concentration of inhibitor and 50 mM Tris/Tris-D 11 (pH 7.5) in 90% H 2 O and 10% D 2 O. The errors shown are the standard deviation from three separate measurements. Compounds 29 and 38 were obtained by virtual high-throughput screening (see Fig. S12–S14)

Article Snippet: Using the crystal structure of ICL1 (PDB ID: ; 1F8I , resolution 2.25 Å), a screen was performed with the InterBioScreen Ltd natural product collection.

Techniques: Concentration Assay, Standard Deviation

Journal: Viruses

Article Title: Identification of Novel Natural Products as Effective and Broad-Spectrum Anti-Zika Virus Inhibitors

doi: 10.3390/v11111019

Figure Lengend Snippet: Initial screening of a natural product library for identification of potential anti-ZIKV inhibitors.

Article Snippet: A plaque reduction inhibition assay was carried out to measure the inhibitory activity of natural products in a natural product collection library from MicroSource Discovery Systems (Gaylordsville, CT, USA) against infections of ZIKV and DENV, as previously described [ , , , ].

Techniques: Inhibition, Activity Assay